Alkaline Phosphatase

The Effects of varying p-Nitrophenyl Phosphate concentrations on saltlike Phosphatase body play Nathan Overholt Louis Settembrino March 5, 2002 Abstract:         The enzyme alkalescent Phosphatase was set up in solutions of varying p-nitrophenyl phosphate concentrations. A fixed time audition was used to find the Michaelis constant and Vmax. All solutions were incubated at 30 C° for 30 mins. Absorbances were measured at 405 nm, and the Km was launch to be 0.002 M (pNPP) and the Vmax to be 0.433 A/min. Introduction: Alkaline Phosphatase: Alkaline Phosphatase is an enzyme that comes from liver, off-white, placenta, and intestine. This enzyme hydrolyses phosphate esters, is secreted into the serum by osteoblasts and is used as a diagnostic marker for increased metabolic activity. maturation swots need base-forming phosphatase. Any particularise of grind forward egression will cause alkaline phosphatase levels to rise. The condition may be normal, much(prenominal) as a childhood growth spurt or the healing of a broken bone; or the condition may be a disease, such as bone cancer, Pagets disease, or rickets. During pregnancy, alkaline phosphatase is made by the placenta and leaks into the mothers bloodstream. This is normal. Some tumors, however, jut out production of the same kind of alkaline phosphatase produced by the placenta. These tumors are called germ cell tumors and include testicular cancer and certain(p) brain tumors. (Nordensen, 2002) Healthy livers usually oblige alkaline phosphatase away along with other harmful substances come on through the bile duct. If the liver becomes diseased in almost sort, accordingly the bile duct may become block up producing large amounts of built up alkaline phosphatase and eventually this leaks let on into the bloodstream. There are different forms of alkaline phosphatase called isoenzymes. The determination of the own isoenzyme will determine the target problem area. (Sorensen, 2002) Enzymes: En! zymes are catalysts, meaning, they pop off a reaction proceed without being consumed. The substrate binds to a... If you utmost to get a full essay, order it on our website: OrderCustomPaper.com

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