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Wednesday, February 5, 2014

growth curve

Measurement of Growth Name : LEVENT Surname : DEMiRAY Student ID# : 140104012 surgical incision : molecular BIOLOGY AND GENETICS Date of Experiment : 27.02.2008 precis In the first science testing groundoratory section of semester, affair was measurement of microbial appendage. At the beginning, protester gave brief closely six measurement way of microbial growth and var. of microbial growth. The most commonly use measurement techniques argon serial dilution and colony counting, microscopic mobile phone counts, tissue layer filtration, turbidity, mass determination and metabolic activity. In this lab 2 of them serial dilution and colony counting and turbidity were employ only. In serial dilution and colony counting which is also named as a viable cell counts, concentrated samples are dilute by serial dilution and then diluted samples are plat ed on rich people complete media. There are two metal plate methods, spread plating and pour plating. They both have close to pros and cons. Moreover turbidity is based on the diffraction or deal out of light by bacteria. To measure absorbance rate spectrophotometer was used. The purpose of this turn up is learning measurement methods of microbial growth completely and practicing these techniques efficiently. Besides, plotting the graph of growth curve is one of the other purposes of this experiment. Introduction In the laboratory, under convenient circumstances, a growing bacterial earth doubles at regular intervals. Growth is by geometrical progression: 1, 2, 4, 8, etc. or 20, 21, 22, 23.........2n (where n = the number of generations). This is called exponential function function growth. In fact, exponential growth is only part of the bacterial life cycle, and not characteristic of the normal recipe of growth of bacteria in nature (1). (2). ! Figure-1 As it clear be seen from the figure 1 the population of bacteria in a...If you desire to get a full essay, order it on our website: OrderCustomPaper.com

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